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... -20°C -check stock of protease inhibitor !!!! -stored samples are: method trypsin/EDTA & scraping Trypsin/EDTA + scraping EDTA/EGTA & scraping EDTA/EGTA +scraping Scraping in LB Scraping in PBS cell fraction... 2x 15 sec. in BIO -one EDTA/EGTA has been sonicated 10sec before ... was added -used protocols are : trypsin/EDTA , ( TC005 ) EDTA/EGTA, ( TC004 ) scraping, (...
Tags:
test different cell harvest methods
http://whx.livejournal.com/5578.html
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Published: 1 year, 2 months ago (Fri, 24 Oct 2008 06:43:10 PDT); 1568 bytes
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