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... G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted “watermarks” at intergenic sites known to tolerate transposon insertions. Overlapping “cassettes” of 5 to 7 kb, assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate...
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http://toxa-musiyenko.livejournal.com/16459.html
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Published: 1 year, 5 months ago (Sat, 26 Jan 2008 14:45:56 PST); 3083 bytes
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